dear editor,<br /><br />unfortnately, my supervisor asked me to revise a few sentenses of the paper you edited before. It is only a few sentences. <br />Could you check it for me? I greatly appreciate your help<br /><br />
<p>1. Our study aims to unveil the release kinetics of nano-ZnO from sunscreen in comparison to industrial-type nano-ZnO particles, with their toxicity significance determined by analyzing both industrial and ZnO sunscreen as measured by algae growth inhibition during the exposure time, concentrations, and the types of nano-ZnO.</p>
<p>&nbsp;2. The extracted material was confirmed as ZnO and all the elemental compositions were the same with those from industrial ZnO (Fig. 1), as indicated by the analysis using energy dispersive spectroscopy (EDS).</p>
<p>&nbsp;3. The ZnO wavelength peak did not interfere as confirmed by measuring ZnO concentrations (both in the form of industrial and extracted particles from ZnO sunscreen) in the water, which showed the absorbance peak wavelength of 395 nm using a DU<sup>&reg;</sup> 720 UV/Vis spectrophotometer (Beckman Coulter, DU<sup>&reg;</sup> 720, Pasadena, CA). The absorbance peak was confirmed using five different concentrations of nano-ZnO in a range of 350&ndash;400 nm (R<sup>2</sup> = 0.998).</p>
<p>&nbsp;4. Statistical analysis: All experiments were triplicated and expressed as a mean &plusmn; SEM (standard error of means). Statistical analysis was carried out using student&rsquo;s <em>t</em>-test and confirmed statistical significance at p &lt; 0.05.</p>
<p>&nbsp;5. The growth inhibition of <em>T. pseudonana</em> may depend on the types of nano-ZnO (i.e., industrial- and sunscreen-extracted nano-ZnO) at a constant concentration. The growth inhibition showed to be significant to the exposure time, whereas little effect of nano-ZnO concentration was observed on the extent of toxicity (Fig. 2).</p>
<p>&nbsp;6. While more studies concerning the size effects on toxicity are to be done, our preliminary results showcase that the types of nano-ZnO and the longevity of nano-ZnO to the exposure attributed to the toxicity.</p>
<p>&nbsp;7. While modeled and detected concentrations of nano-ZnO in water streams were reported to be less than 10 mg/L (Gottschalk et al., 2013), our preliminary study raises concern on the significant release of nano-ZnO due to the potential toxicity from accumulation of nano-ZnO. This is in good agreement with recent results on the evaluation of nanoparticles during drinking water treatment where 48 to 99% ranges of ZnO still remain in finished water after conventional treatment (Abbott Chalew et al., 2013).</p>
<p>&nbsp;8. Fig. 1 EDS image of the industrial-derived (a) and consumer products-derived nano-ZnO (b). [Note: Since the pretreatment process requires ultra-thin coating of electrically conducting gold/palladium, the Pd element is shown in the EDS analysis].</p>
<p>&nbsp;9. Note that the large and spherical particles are zinc oxide</p>
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